The paper "Bioanalytical Techniques on Tissue Culture" is an outstanding example of a term paper on biology. Tissue culture is the extraction of tissues and cells from an animal or plant so as to be grown in an artificial environment provided with the necessary conditions24, p13. It is an area of research that has grown in popularity over the years and has become very fundamental in the study of cell biology. The present review paper exhaustively addresses the aseptic technique of culturing mammalian tissues and cells with the aim of understanding its application free of ‘ contaminants’ .
The aseptic technique is selected from among others for its deep concern in fostering high levels of safety. Various methods have been proposed in conducting an aseptic technique of mammalian tissue culture. In the views of 14, p11, aseptic technique can be successfully carried out by developing cultures as either ‘ adherents/monolayers; suspensions or three-dimensionally has grown’ . These three methods of developing mammalian tissue cultures aseptically have seen their fair share of praise from a number of proponents. The present review report critically analyses these three methods as they have been applied. The review paper has been keen to conclude that the aseptic technique of mammalian tissue and cell culture is the most relevant in today’ s molecular and cell biology due to its insistence on ensuring that both the environment and equipment are free from contamination22, p26.
This bio-safety is also ensured by the application of other procedures including ‘ trypsinizing, freezing/thawing and sub-culturing’ . The cultivated tissues and cells/organs have found their use in clinical and regenerative medicine as well as in research15, p111. Objectives To identify the various bio-techniques used in mammalian tissue and cell culture, To review one such bio-technique used in the said field, To pinpoint the key aspects of the identified bio-technique in mammalian life, To analyze the various methods applicable to the identified bio-technique, and To describe the key application areas of cultivated tissues and cells/organs Key Findings The present review paper has come up with the following key findings: The aseptic technique of mammalian tissue and cell culture is successful in maintaining a ‘ safe’ bank of tissues, cells, and organs for the perpetuation of mammalian life, The technique is enabled by three methods of culturing namely: adherent, suspension, and three-dimensionally grown, The success of the aseptic technique depends on keeping the “ environment, equipment, and personal ‘ aura’ ” free from contamination, and Cultivated tissues, cells and organs through aseptic techniques are applicable in a wide range of medical conditions including clinical and generative medicine, research. Keywords Aseptic Technique – free from any form of contamination, abiotic or biotic Adherent/Monolayer cultures – cultures requiring a surface or substratum to attach themselves Suspension cultures – cultures which don’ t require anchorage but are grown suspended in space Three-dimensionally growing cultures – cultures that grow in spheroids assuming 3D shapes i. e.
length, width, and depth Trypsinising – mechanically detaching adherent cells from the surface of the culture vessel Sub-culturing – transferring primary culture cells into secondary cultures to restart fresh growths. Cells in vitro – cells in artificial environments such as ‘ Petri-dishes, flasks, and bioreactors’ 23, p17. INTRODUCTION As is explained in the extant literature, tissue culture is synonymous with ‘ cell culture, stem culture, or organ culture’ , all referring to the extraction of body segments from an organism in order to cultivate them in artificial environments16, p76. The technique is a twentieth-century discovery, during which time the products were only used for study purposes alone.
As time went by, tissue culture techniques evolved rapidly to what they are today: “ methods of mass production of potent tissues and cells capable of giving rise to independent cell lines” 4, p33. It is in the contention that tissue culture techniques have opened a very hopeful window in cell biology as well as microbiology in the management of life.
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