Mitochondria and Cellular Respiration – Lab Report Example

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The paper "Mitochondria and Cellular Respiration  " is a worthy example of a lab report on biology. Mitochondria are double-membrane and rods shaped organelles found in the cells of both animals and plants and are responsible for various metabolic functions within the cells. Mitochondria play a vital role in providing the necessary biological energy for the cell through enzymatic oxidation of the substrates of the Krebs’ s cycle. The mitochondrion is made up of outer membrane, inner membrane, intermembrane space, and matrix. The outer membrane consists of the same amounts of proteins and phospholipids as well as a large number of porins which are specialized proteins.

The outer membrane is permeable to ions, nutrients and energy molecules such as ADP and ATP (Abdrakhimova et al, 2011). The inner membrane of the mitochondrion is complex and consists of a number of folds known as the cristae which are important since it increases the surface area within the organelle. The increased surface area within the organelle coupled with the presence of various proteins within the inner membrane is crucial in aiding various enzymatic reactions including the production of ATP.

However, unlike the outer membrane, the inner membrane is strictly permeable to ATP, Oxygen, and movement of metabolites across the membrane (Jones, 2013). The space between the outer and the inner membranes is referred to as the intermembrane space and its composition is similar to that of the cytoplasm. The matrix which is the fourth component of the mitochondrion is made up of a complex mixture of enzymes and proteins which are important in the synthesis of the ribosome, ATP, mitochondrial DNA and tRNAs (Miles et al, 2014).

Succinate dehydrogenase is one of the enzyme complex bound to the inner membrane of the mitochondrion. Succinate dehydrogenase plays a vital role in Krebs’ s cycle since it catalyzes the oxidation of Succinate to fumarate within the inner membrane (Gorbacheva et al, 2013). Objective In this experiment, we examined the intracellular location of the Succinic dehydrogenase. As outlined above, succinic dehydrogenase is enzymes involved in redox reactions during cellular respiration by catalyzing the conversion of succinic acid to fumaric acid in the Kreb’ s Cycle as shown below. Through measurements of FADH2 produced in the reaction, it is possible to determine the intracellular location of Succinic Dehydrogenase.

Since measuring FADH2 directly is hard, this compound can be coupled with a second reaction that results in a colored product that can be measured using a spectrophotometer. Fractionation of Cauliflower Cells The top 2-3 mm off the heads of fresh cauliflower were shoved to give 25g of tissue then blended for about 10 seconds in 20mL cold grinding buffer (0.4M sucrose; 6mM KH2PO4; 14mM K2HPO4, pH7.2. The slurry was then poured through 4 layers of muslin cloth and the filtrate collected in a tube.   The blender was then rinsed with 10mL cold grinding buffer, filtered and added to the tube to form the homogenate.

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