Animal Endocrine Pancreatic Physiology – Lab Report Example

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The paper “ Animal Endocrine Pancreatic Physiology” is an informative example of a lab report on biology. There are a number of experiments developed to establish glucose metabolism. The standard test for the pancreases to secrete insulin is the glucose test. The aim of the experiment was to introduce the concept of Intravenous Glucose Tolerance Test (IVGTT), hence demonstrate how the pancreases of a sheep respond to a glucose load. Additionally, the practical aimed at determining the influence of different nutritional planes on IVGTT results. The clearance rate for a given load of glucose is directly proportional to the secretion of insulin and hence indicates the ability of the pancreas to respond to hyperglycemia by secreting insulin. Materials/MethodsEach student group was provided with the following materials; a sheep with an indwelling venous catheter and safe-site port, syringes, needles, and heparin saline as well as glucose solution (50% w/v).

Also issued at the class level were advantage Glucose Meter and Advantage II glucose detection strips. The practical involved two different treatments. Three sheep fed on a cereal grain-based diet that is a high plane of nutrition and three on roughage low on nutrition.

Different groups worked on each of the six sheep by conducting an IVGTT. The sheep received an injection of 0.4 g/Kg (live weight) glucose intravenously in the form of a 50% glucose solution for a period of about 1 to 2 minutes. We took blood samples before and after the injection. Each group through the indwelling intravenous Cather’ s did a collection of blood from the sheep. We noted the diet the sheep fed on. The collection of blood involved injecting a 5 ml syringe containing heparinized saline to the safe-site port.

To ensure that fresh blood was in the Cather, we withdrew a dead space from the Cather tube. For uninterrupted blood flow, we moved sheep’ s heads in case the tubing pressed itself against something inside the sheep. Additionally, an injection of heparinized saline into the Cather cleared any clots that may have occurred, and then syringe withdrawal followed. We withdrew the 5 ml syringe containing a mixture of blood and heparinized saline following the Cather being full of fresh blood, and then attached a collection of 2ml blood.

We withdrew the blood into the syringe and a 5ml syringe full of pure heparinized saline attached to the safe-port. We recorded the time of withdrawing the blood. An injection of 1 to 2 mills of heparinized saline into the Cather followed to ensure that the tube had no blood. We then removed the syringe. From the main menu bar, we selected the “ Run Glucose Test” and inserted a glucose detection strip when we received a prompt command. To avoid disruption of future tests we closed the container of the detection strips within 30 seconds.

Another prompting led to the use of a Pasteur pipette to withdraw blood from the heparin zed tube and a sufficient single drop of blood placed inside the yellow notch on the detection strip. A touch on the drop of blood to the yellow strip made it flow across the yellow area covering it completely. We took a measurement of the blood glucose from the glucose meter.

Bibliography

Ferrannini, E. & Pilo, A. (1979). A pattern of Insulin Delivery after Intravenous Injection in man and its

Relation to plasma glucose Disappearance. Journal of Clinical Investigation. Vol. 64(1), 243-254.

Roden, M. (2007). Clinical diabetes research methods and techniques. Chichester, West

Sussex, England, John Wiley & Sons. http://site.ebrary.com/id/10297841.

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