Concentration-Effect Curves for Histamine-Induced Effect of Guinea-Pig Ileum in the Presence and Absence of the Concentrations of Mepyramine – Lab Report Example

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The paper “ Concentration-Effect Curves for Histamine-Induced Effect of Guinea-Pig Ileum in the Presence and Absence of the Concentrations of Mepyramine” is a potent variant of a lab report on biology.   From the concentration-effect curves, the dose that is necessary to produce a response that is equal to 50% of the maximum response induced values for mepyramine were determined to be 1.25x10-6. This means that the maximum response produced by histamine was depressed thus it means they were ineffective. It is important in order to reduce the negative effects caused by histamine at these levels.

This concentration is one of the most effective long-term measures of dosage usage. The ED50 values for mepyramine after histamine-induced effect of guinea-pig ileum of 1X10-9. Maximal contractions induced by the histamine-induced was using mean + standard error of the mean. At 1X10-9 the response produced histamine was not significant although there is the inducement of short circuit current. The main response to none effectiveness of mepyramine is because histamine was blocked. Histamine influences the mepyramine exchange process thus acting as receptors This supported by the constant oscillation of the curve up and down as the concentration changes.   Then calculate equipotent concentration ratios (ECR)- this is where the molar concentration of Histamine is divided by mepyramine at the point where a response is equal 50% of the maximum response induced values for mepyramine Equipotent concentration ratio = = 12.5 Conc Histamine Mepyramine 1 x 10-9 M ECR the log of the (ECR-1) negative log of the antagonist 0.000000003 9 0.3333 -0.48 8.52   0.000000005 12 0.2000 -0.70 8.30   0.000000010 24 0.1000 -1.00 8.00   0.000000030 40 0.0333 -1.48 7.52   0.000000050 46 0.0200 -1.70 7.30   0.000000100 50 0.0100 -2.00 7.00   0.000000300 54 0.0033 -2.48 6.52   0.000000500 54 0.0020 -2.70 6.30               0.000000125           Equipotent concentration ratio The negative log of the antagonist concentration (x-axis) against the log of the (ECR-1) The slope parameter is -1.064 and the intercept parameter is 9.438 which is pM2 value.

The pM2 value is the negative logarithm of the molar concentration of the antagonist which will reduce the effect of a multiple-dose of the agonist (x) to that of a single dose The estimated regression equation for the data is Y = 9.438 – 1.064 X. The slope parameter is the average increment in the dependent variable when the dependent variable increases by one unit. The graph slopes from the upper left of the scatter plot to lower right suggesting a negative relationship between the two variables. 1X10-8 Concentration-effect curves for the histamine-induced effect of guinea-pig ileum in the presence and absence of concentration of mepyramine From the concentration-effect curves, the dose that is necessary to produce a response that is equal 50% of the maximum response induced values for mepyramine were determined to be 2.49x10-7.

This means that the maximum response produced by histamine was depressed thus it means they were ineffective. It is important in order to reduce the negative effects caused by histamine at these levels. This concentration is one of the most effective long-term measures of dosage usage.

The ED50 values for mepyramine after histamine-induced effect of guinea-pig ileum of 1X10-8. Maximal contractions induced by the histamine-induced were using mean + standard error of the mean. At 1X10-8 the response produced histamine was not significant although there is the inducement of short circuit current. The main response to none effectiveness of mepyramine is because histamine was blocked. Histamine influences the mepyramine exchange process thus acting as receptors as it produces a dose-related contraction with similar potency. This supported by the constant oscillation of the curve up and down as the concentration changes.  

References

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Nijkamp, F.P. & Van Ree, J.M., 1980. Effects of Endorphins On Different Parts Of The Gastrointestinal Tract Of Rat And Guinea-Pig In Vitro. Rudolph Magnus Institute for Pharmacology, University of Utrecht, Utrecht, the Netherlands

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